Crosslinked histone octamer as a model of the nucleosome core.
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چکیده
منابع مشابه
Crosslinked histone octamer as a model of the nucleosome core.
When histones in chromatin core particles were crosslinked with dimethylsuberimidate, the resulting particles had properties closely similar to those of native core particles. A crosslinked octameric histone complex was isolated from these particles under nondenaturing conditions. Upon reaction with DNA, this octameric protein folded the DNA into a structure closely resembling that of native co...
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should be compared with figure 2a in (2).These maps are "before" and "after" solvent flattening respectively, and clearly demonstrate that our noise filtering procedure did not introduce artifacts into the map. 4) Klug et al., seem to have overlooked the information presented (2, p. 547) and concluded that we have incorrectly chosen the protein boundaries. On the contrary, 95 percent of the bou...
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Adenosine 5'-triphosphate (ATP)-dependent chromatin remodeling enzymes play essential biological roles by mobilizing nucleosomal DNA. Yet, how DNA is mobilized despite the steric constraints placed by the histone octamer remains unknown. Using methyl transverse relaxation-optimized nuclear magnetic resonance spectroscopy on a 450-kilodalton complex, we show that the chromatin remodeler, SNF2h, ...
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The rate of transcription through the nucleosome, the fine structure of the nucleosomal barrier, and the fate of the nucleosome during transcription at different salt concentrations were analyzed using linear 227-base pair mononucleosomal templates containing a uniquely positioned nucleosome core. At lower ionic strength (30 mm NaCl), the nucleosome constitutes a strong barrier for SP6 RNA poly...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1977
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.74.7.2780